anti reca1  (Bio-Rad)

Journal: CNS Neuroscience & Therapeutics

Article Title: Targeting blood brain barrier—Remote ischemic conditioning alleviates cognitive impairment in female APP / PS1 rats

doi: 10.1111/cns.14613

Figure Lengend Snippet: Antibody information.

Article Snippet: RECA1 , 1:100 , / , Santa Cruz , M , AB_1128987 , Sc‐71,958.

Techniques:

Journal: CNS Neuroscience & Therapeutics

Article Title: Targeting blood brain barrier—Remote ischemic conditioning alleviates cognitive impairment in female APP / PS1 rats

doi: 10.1111/cns.14613

Figure Lengend Snippet: Remote ischemia conditioning protected vascular integrity in the frontal–parietal cortex of APP/PS1 OVX rats. (A, a1‐a2) Representative images showing immunofluorescence staining for RECA1 (red) and the vessel density was measured based on RECA‐1‐positive structures in the cortex. Vascular integrity was assessed according to RECA‐1‐positive structures that were thresholded into three‐pixel areas‐100–2000 (a1), 20,001–5000 (no significance between groups, statistic data was not shown), and 5001–infinity (a2) using Fiji/Image software. Data were expressed as means ± SEM. (B) Representative photos of double immunofluorescence staining for ZO‐1 with RECA1 and occludin with RECA1. Quantitative analysis of ZO‐1 (b1) and occludin (b2) fluorescence intensity expressed as fold changes vs. WT group. n = 5–6 for B. magnification 40 ×, scale bar 50 μm. (C) TEM results showed vascular ultrastructure in the rat cortex. Representative images of microvascular morphological changes are shown. Microvessels are compressed and narrowed by swollen astrocytic endfeet in APP/PS1 rats, while the astrocytic endfeet swelling is mild and the residual vascular lumen was well‐preserved in RIC rats. A, astrocyte; BL, basal lamina; EC, endothelial cell; L, capillary lumen; TJ, tight junction. (D) Representative images of IgG (green) among different groups. The quantitative analysis of IgG fluorescence intensity expressed as fold changes vs. WT group is shown in (d1). (E) The changes in the content of Evans blue in different groups and different ages are shown. Values are means ± SEM of determinations from each group. N = 6 for D; n = 6 for E. ns., no significance.

Article Snippet: RECA1 , 1:100 , / , Santa Cruz , M , AB_1128987 , Sc‐71,958.

Techniques: Immunofluorescence, Staining, Software, Double Immunofluorescence Staining, Fluorescence

Journal: CNS Neuroscience & Therapeutics

Article Title: Targeting blood brain barrier—Remote ischemic conditioning alleviates cognitive impairment in female APP / PS1 rats

doi: 10.1111/cns.14613

Figure Lengend Snippet: Effects of remote ischemia conditioning vascular‐related factors in the frontal–parietal cortex of OVX APP/PS1 rats. (A) Representative photographs of double immunofluorescence of PDGFRβ with RECA1. Surface analysis showed the total PDGFRβ (a1), and vessel‐associated PDGFRβ (a2), n = 7–8. (B, b1, b2) Western blot and data analysis for PDGFRβ and VCAM1. N = 4–5. Magnification 40×, scale bar 50 μm.

Article Snippet: RECA1 , 1:100 , / , Santa Cruz , M , AB_1128987 , Sc‐71,958.

Techniques: Immunofluorescence, Western Blot

Journal: CNS Neuroscience & Therapeutics

Article Title: Targeting blood brain barrier—Remote ischemic conditioning alleviates cognitive impairment in female APP / PS1 rats

doi: 10.1111/cns.14613

Figure Lengend Snippet: Effects of remote ischemia conditioning on astrocyte morphology and the interaction with vascular cells in the frontal–parietal cortex of OVX APP/PPS1 rats. (A) Representative photographs of double immunofluorescence staining of GFAP (green) with RECA1 (red) and the surface analysis aided by Imaris 9.5 software. Quantitative analyses of vascular‐associated GFAP in (a1) and total GFAP intensity is shown and (a2), respectively. (B) Hyalinization analysis (Opaque and Transparent) on vascular cells showed that astrocytes were mainly distributed inside the vascular lumen in the APP/PS1 rats. (C) GFAP mRNA expression by RT‐qPCR. (D) GFAP protein expression by Western blot analysis. (E) Representative photographs of astrocytes by TEM. Enlarged mitochondria from each group are shown in the second column. C, cytoplasm; M, nucleus membrane; Mit, mitochondria; N, nucleus. (F) GFAP was detected in serum by EILSA. Magnification 40×, scale bar 50 μm.

Article Snippet: RECA1 , 1:100 , / , Santa Cruz , M , AB_1128987 , Sc‐71,958.

Techniques: Double Immunofluorescence Staining, Software, Expressing, Quantitative RT-PCR, Western Blot, Membrane

Journal: CNS Neuroscience & Therapeutics

Article Title: Targeting blood brain barrier—Remote ischemic conditioning alleviates cognitive impairment in female APP / PS1 rats

doi: 10.1111/cns.14613

Figure Lengend Snippet: Remote ischemia conditioning upregulated GLUT1 protein expression in the frontal–parietal cortex of OVX APP/PS1 rats. (A) Representative photographs of triple immunofluorescence staining of GLUT1 (green), GFAP (blue), and RECA1 (red) and the surface analysis. (a1) Quantitative analysis of the fluorescent intensity of GLUT1+ staining. Pearson's R ‐value analysis (a2) and colocalization (a3) between the two stained proteins for GLUT1/RECA1. N = 6–8, scale bar 50 μm, magnification 40×.

Article Snippet: RECA1 , 1:100 , / , Santa Cruz , M , AB_1128987 , Sc‐71,958.

Techniques: Expressing, Immunofluorescence, Staining

Journal: CNS Neuroscience & Therapeutics

Article Title: Targeting blood brain barrier—Remote ischemic conditioning alleviates cognitive impairment in female APP / PS1 rats

doi: 10.1111/cns.14613

Figure Lengend Snippet: Remote ischemia conditioning suppressed Aβ production in the frontal–parietal cortex of OVX APP/PS1 rats. (A) Representative photographs of BACE1 (green) and RECA1 (red). Quantitative analysis of the fluorescence intensity of BACE1+ staining in the hippocampal CA1 (a1) and cortex (a2). Scale bar 50 μm, magnification 40×. (B) Representative photographs of ADAM10 (α‐Secretase) immunofluorescence staining (green). DAPI counterstaining of the nucleus. Quantitative analysis of the fluorescent intensity of ADAM10 in the cortex (b1), and hippocampal CA1 (b2). Scale bar 50 μm, magnification 40×. (C, c1–c4) Western blot analysis showed changes of nicastrin, presenilin 1, PEN2, and presenilin 2. Values are expressed as means ± SEM of determinations from each group. The typical photographs of 6E10 immunofluorescence staining (red) are shown in (D). Quantitative analysis of the fluorescent intensity of 6E10 in the cortex (d1), and hippocampal CA1 (d2). Scale bar 200 μm, magnification 10× for the left column; and scale bar 50 μm, magnification 40× for the right column. (E, F) The level of Aβ1‐42 and Aβ1‐40 in the cortex was detected by ELISA assay. N = 5–8, White Box in subfigure A. Hip CA1, hippocampal CA1.

Article Snippet: RECA1 , 1:100 , / , Santa Cruz , M , AB_1128987 , Sc‐71,958.

Techniques: Fluorescence, Staining, Immunofluorescence, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells

doi: 10.1016/j.isci.2023.108306

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-RECA1 , Bio-Rad , Cat#MCA970R; RRID: AB_323297.

Techniques: Recombinant, Blocking Assay, Software, Microscopy, Laser-Scanning Microscopy